供應磺胺二甲基嘧啶試劑盒

磺胺二甲基嘧啶酶聯免疫快速檢測試劑盒(SM₂-ELISA)1 概要磺胺二甲基嘧啶（SM₂）是應用廣泛的抗生素，對于畜禽和動物水產品養殖過程中疾病的控制和治療起到重要作用。研究證明SM₂具有潛在的毒副作用，在動物機體中長期存在的SM₂會發生多種代謝作用：代謝物主要為N4位置上的酰化化合物，N4位置上的葡萄糖化合物，和脫芳氨基化合物。若與亞硝酸鹽共同攝取能夠促進生成脫芳氨基代謝物的形成，該物質有一定的誘導突變作用。我國農業部第235號文件規定了其殘留量為在牛奶中為25μg/kg，由于磺胺二甲基嘧啶的廣泛應用，對于其他動物組織檢測也有必要。由于高效液相色譜等儀器分析方法樣本前處理盒測定的繁瑣和費用高，推廣使用受到了限制。本試劑盒可用于定量、定性檢測魚、蝦樣本，動物組織，禽蛋中和牛奶樣本中的SM₂殘留。與儀器分析技術相比較具有快速，簡便，準確和靈敏度高，特異性好等優點。操作時間僅需1小時左右，同時能夠實現多個樣本的同時測定。在提供準確結果的同時，減少了工作強度。2 檢測原理本試劑盒采用間接競爭抑制法檢測樣本中的SM₂。在微孔板上預包被SM₂抗原，樣本或標準品中的SM₂殘留和包被抗原競爭結合抗SM₂的單克隆抗體，經過孵育后，洗滌未與包被抗原結合的抗SM₂的單克隆抗體，加入抗鼠單抗的二抗-HRP，經過孵育后，洗滌游離的二抗-HRP后用H₂O₂-TMB底物系統顯色，樣本吸光度值與其所含SM₂的成負相關，與標準品溶液的比較即可得出SM₂含量。3 試劑盒組份每個試劑盒中的試劑可進行48個測量（包括標準測定），試劑盒組分：                                                                                      包被有SM₂抗原的微孔條······················································· 48孔（8孔/條×6條）板架················································································· 1塊（8×12）SM₂標準品溶液·················································································· 4瓶（0.3ml/瓶）分別為0號：0ppb；1號：0.4ppb；2號：2ppb；3號：10ppb。抗SM₂單克隆抗體濃縮液（100×）················································· 1瓶（50μL/瓶）二抗-HRP濃縮液（100×）······························································· 1瓶（50μL/瓶）抗體稀釋液························································································· 1瓶（10ml/瓶）底物溶液A··························································································· 1瓶（4 mL/瓶）底物溶液B···························································································· 1瓶（4 mL/瓶）終止液（含2 M的硫酸）··································································· 1瓶（4 mL/瓶）20×濃縮洗滌液··················································································· 1瓶（25 mL/瓶）10×樣品復溶液··················································································· 1瓶（10 mL/瓶）封板膜··································································································· 3張說明書··································································································· 1份 4 另外所需試劑和儀器4.1 試劑--甲醇 （分析純）--正己烷（分析純）4.2 設備--微孔板酶標儀（450 nm）--恒溫箱   （37℃）--天平（精度0.01 g）--勻漿器--振蕩器--超聲波清洗器（工作頻率不小于40KHZ）--離心機和離心管--旋轉蒸發器或氮氣吹干儀--可調式0.5μL-20 μL， 20 μL-200 μL 和100 μL-1000 μL 微量移液器--多道移液器4.3 耗材10 mL圓底帶蓋離心管

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