南湖新聞網訊（通訊員 宗茂）近日，華中農業大植物科學技術學院農藥毒理學及有害生物抗藥性團隊研究成果以“Redox and Near-Infrared Light-Responsive Nanoplatform for Enhanced Pesticide Delivery and Pest Control in Rice: Construction, Efficacy, and Potential Mechanisms”為題在ACS Applied Materials & Interfaces上發表。研究采用了一種創新的納米平臺，成功實現了對水稻重要害蟲褐飛虱防控藥劑的高效智能遞送，進一步闡明了其增效機制。
褐飛虱（Nilaparvata lugens）是我國水稻生產上的重要害蟲，對水稻產量和品質造成嚴重危害。基于此，研究團隊開發了一種鑲嵌硫化銅的有機二氧化硅納米復合載體，將其應用于新煙堿類殺蟲劑烯啶蟲胺的控制釋放。硫化銅納米顆粒可將近紅外光能轉化為熱量，從而實現農藥的快速釋放和協同增效效應。同時，有機二氧化硅納米顆粒具有豐富的孔隙結構和較大的比表面積，可高效負載農藥分子，并對害蟲體內谷胱甘肽作出響應。硫化銅可實現快速光熱轉化，納米平臺具有較高的光熱轉化率，在近紅外光的激發下，與烯啶蟲胺原藥相比，納米平臺對褐飛虱田間種群和抗藥性種群的毒力均顯著提升。進一步發現納米平臺可顯著抑制褐飛虱抗藥性種群中P450基因（NlCYP6ER1，NlCYP6AY1，NlCYP4C76）的表達，進而提高其對殺蟲劑的敏感性，研究結果為害蟲抗藥性的治理提供了新思路。該納米平臺在有害生物綠色高效防控方面具有巨大應用潛力。
植物科學技術學院碩士研究生宗茂為論文第一作者，何順副教授為論文通訊作者，李建洪教授、萬虎教授和馬康生副教授參與了項目的指導。該研究得到湖北省重點研發計劃和國家自然科學基金等項目資助。
審核人：李建洪
【英文摘要】
The brown planthopper （BPH）， Nilaparvata lugens （St?l）， is a major rice pest in various Asian countries, causing significant negative impacts on rice yield and quality. In this study, we developed a novel nanoplatform （NIT@MON@CuS） for pesticide delivery that responds to redox and near-infrared light stimuli. The nanoplatform consisted of CuS nanoparticles with mesoporous organic silica （MON）， loaded with nitenpyram （NIT）。 With an average size of 190 nm and a loading efficiency of 22%, the NIT@MON@CuS exhibited remarkable thermal response in the near-infrared region, demonstrating excellent photothermal conversion ability and stability. In vitro release kinetics demonstrated that rapid released nitenpyram under near-infrared light and glutathione conditions, facilitating a satisfactory temperature increase and accelerated drug release. The NIT@MON@CuS treated group exhibited a higher mortality of N. lugens, increasing from 62% to 88% compared to the group treated with nitenpyram technical after 96 hours. Bioassay revealed that NIT@MON@CuS significantly enhanced nitenpyram toxicity by more than 1.4-fold against both laboratory insecticide-resistant and field strains of N. lugens. Furthermore, RT-qPCR results demonstrated that MON@CuS had the capability to reduce P450 gene expression thereby improving the sensitivity of N. lugens to insecticides. These findings suggest that MON@CuS hold great potential as an intelligent pest control platform, offering a sustainable and efficient approach to protect crops against pests.
論文鏈接：https://pubs.acs.org/doi/abs/10.1021/acsami.3c08413
日期：2023-10-04
褐飛虱（Nilaparvata lugens）是我國水稻生產上的重要害蟲，對水稻產量和品質造成嚴重危害。基于此，研究團隊開發了一種鑲嵌硫化銅的有機二氧化硅納米復合載體，將其應用于新煙堿類殺蟲劑烯啶蟲胺的控制釋放。硫化銅納米顆粒可將近紅外光能轉化為熱量，從而實現農藥的快速釋放和協同增效效應。同時，有機二氧化硅納米顆粒具有豐富的孔隙結構和較大的比表面積，可高效負載農藥分子，并對害蟲體內谷胱甘肽作出響應。硫化銅可實現快速光熱轉化，納米平臺具有較高的光熱轉化率，在近紅外光的激發下，與烯啶蟲胺原藥相比，納米平臺對褐飛虱田間種群和抗藥性種群的毒力均顯著提升。進一步發現納米平臺可顯著抑制褐飛虱抗藥性種群中P450基因（NlCYP6ER1，NlCYP6AY1，NlCYP4C76）的表達，進而提高其對殺蟲劑的敏感性，研究結果為害蟲抗藥性的治理提供了新思路。該納米平臺在有害生物綠色高效防控方面具有巨大應用潛力。
植物科學技術學院碩士研究生宗茂為論文第一作者，何順副教授為論文通訊作者，李建洪教授、萬虎教授和馬康生副教授參與了項目的指導。該研究得到湖北省重點研發計劃和國家自然科學基金等項目資助。
審核人：李建洪
【英文摘要】
The brown planthopper （BPH）， Nilaparvata lugens （St?l）， is a major rice pest in various Asian countries, causing significant negative impacts on rice yield and quality. In this study, we developed a novel nanoplatform （NIT@MON@CuS） for pesticide delivery that responds to redox and near-infrared light stimuli. The nanoplatform consisted of CuS nanoparticles with mesoporous organic silica （MON）， loaded with nitenpyram （NIT）。 With an average size of 190 nm and a loading efficiency of 22%, the NIT@MON@CuS exhibited remarkable thermal response in the near-infrared region, demonstrating excellent photothermal conversion ability and stability. In vitro release kinetics demonstrated that rapid released nitenpyram under near-infrared light and glutathione conditions, facilitating a satisfactory temperature increase and accelerated drug release. The NIT@MON@CuS treated group exhibited a higher mortality of N. lugens, increasing from 62% to 88% compared to the group treated with nitenpyram technical after 96 hours. Bioassay revealed that NIT@MON@CuS significantly enhanced nitenpyram toxicity by more than 1.4-fold against both laboratory insecticide-resistant and field strains of N. lugens. Furthermore, RT-qPCR results demonstrated that MON@CuS had the capability to reduce P450 gene expression thereby improving the sensitivity of N. lugens to insecticides. These findings suggest that MON@CuS hold great potential as an intelligent pest control platform, offering a sustainable and efficient approach to protect crops against pests.
論文鏈接：https://pubs.acs.org/doi/abs/10.1021/acsami.3c08413
日期：2023-10-04
