portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">設(shè)定移液體積：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">從大量程調(diào)節(jié)至小量程為正常調(diào)節(jié)方法，逆時(shí)針旋轉(zhuǎn)刻度即可；portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">從小量程調(diào)節(jié)至大量程時(shí)，應(yīng)先調(diào)至超過設(shè)定體積刻度，再回調(diào)至設(shè)定體積，這樣可以保證移液器的精確度。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">2.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">裝配移液槍頭：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">將移液槍垂直插入吸頭，左右旋轉(zhuǎn)半圈，上緊即可。portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">（注意：用移液器撞擊吸頭的方法是非常不可取的，長(zhǎng)期這樣操作回導(dǎo)致移液器的零件因撞擊而松散，嚴(yán)重會(huì)導(dǎo)致調(diào)節(jié)刻度的旋鈕卡住。）
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">吸液及放液：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">垂直吸液portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; text-decoration: underline;">portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">吸頭尖端浸入液面portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3mmportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">以下，吸液前槍頭先在液體中預(yù)潤(rùn)洗portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">2portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">～portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">3portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">次確保移液的精度和準(zhǔn)度portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">（因?yàn)槲^內(nèi)壁會(huì)殘留一層“液膜”，造成排液量偏小而產(chǎn)生誤差）；portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋體;">慢吸慢放portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">，以防突然松開溶液吸入過快而沖入取液器內(nèi)腐蝕柱塞而造成漏氣；portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">放液時(shí)如果量很小則應(yīng)吸頭尖端可靠容器內(nèi)壁。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">（使用時(shí)要檢查是否有漏液現(xiàn)象。方法是吸取液體后懸空垂直放置幾秒中，看看液面是否下降。如果漏液，則檢查吸液嘴是否匹配和彈簧活塞是否正常。）
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">4. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">濃度和粘度大的液體：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">會(huì)產(chǎn)生誤差，為消除其誤差的補(bǔ)償量，可由試驗(yàn)確定，補(bǔ)償量可用調(diào)節(jié)旋鈕改變讀數(shù)窗的讀數(shù)來進(jìn)行設(shè)定。（可采用portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋體;">反向移液技術(shù)portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">移取高粘度液體）
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">5portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">吸有液體的移液槍不應(yīng)平放：portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">槍頭內(nèi)的液體很容易污染槍內(nèi)部而可能導(dǎo)致槍的彈簧生銹。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">6portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">移液槍在每次實(shí)驗(yàn)后應(yīng)將刻度調(diào)至最大portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">讓彈簧回復(fù)原型以延長(zhǎng)移液槍的使用壽命。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">7portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">移液槍校正：
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">可用分析天平稱量所取純水的重量并進(jìn)行計(jì)算的方法，來校正取液器，portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1mLportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">蒸餾水portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">20portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">℃時(shí)重portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">0.9982gportant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">8portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋體;">移液器嚴(yán)禁吸取有強(qiáng)揮發(fā)性、強(qiáng)腐蝕性的液體（如，濃酸、濃堿、有機(jī)物等portant; word-wrap: break-word !important;">）portant; word-wrap: break-word !important;">。
portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">9portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">嚴(yán)禁使用移液器吹打混勻液體。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">10portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">. portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">不要用大量程的移液器移取小體積的液體，以免影響準(zhǔn)確度；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">同時(shí)，如果需要移取量程范圍以外較大量的液體，請(qǐng)使用移液管進(jìn)行操作。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">1portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">.portant; word-wrap: break-word !important; text-decoration: underline; font-size: 16px; line-height: 24px; font-family: 宋體;">如液體不小心進(jìn)入活塞室應(yīng)及時(shí)清除污染物；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">定期清潔移液槍外壁，可以用portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">95%portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">酒精或portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px;">60%portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">的異丙醇，再用蒸餾水擦拭，自然晾干。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器使用注意事項(xiàng)
portant; word-wrap: break-word !important; font-size: 14px; font-family: 微軟雅黑, sans-serif; color: rgb(85, 85, 85);">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(1)吸取液體時(shí)一定要緩慢平穩(wěn)地松開拇指，絕不允許突然松開，以防將溶液吸入過快而沖入取液器內(nèi)腐蝕柱塞而造成漏氣。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(2)為獲得較高的精度，吸頭需預(yù)先吸取一次樣品溶液，然后再正式移液，因?yàn)槲⊙宓鞍踪|(zhì)溶液或有機(jī)溶劑時(shí)，吸頭內(nèi)壁會(huì)殘留一層“液膜”，造成排液量偏小而產(chǎn)生誤差。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(3)濃度和粘度大的液體，會(huì)產(chǎn)生誤差，為消除其誤差的補(bǔ)償量，可由試驗(yàn)確定，補(bǔ)償量可用調(diào)節(jié)旋鈕改變讀數(shù)窗的讀數(shù)來進(jìn)行設(shè)定。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(4)可用分析天平稱量所取純水的重量并進(jìn)行計(jì)算的方法，來校正取液器，1mL蒸餾水20℃時(shí)重0.9982g。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(5)portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">移液器portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">反復(fù)撞擊吸頭來上緊的方法是非常不可取的，長(zhǎng)期操作會(huì)使內(nèi)部零件松散而損壞移液器。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(6)移液器未裝吸頭時(shí)，切莫移液。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(7)在設(shè)置量程時(shí)，請(qǐng)注意？旋轉(zhuǎn)到所需量程？數(shù)字清清楚楚在顯示窗中？所設(shè)量程在移液器量程范圍內(nèi)不要將按鈕旋出量程，否則會(huì)卡住機(jī)械裝置，損壞了移液器。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(8)移液器嚴(yán)禁吸取有強(qiáng)揮發(fā)性、強(qiáng)腐蝕性的液體（如，濃酸、濃堿、有機(jī)物等）。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(9)嚴(yán)禁使用移液器吹打混勻液體。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">(10)不要用大量程的移液器移取小體積的液體，以免影響準(zhǔn)確度。同時(shí)，如果需要移取量程范圍以外較大量的液體，請(qǐng)使用portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">移液管portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">進(jìn)行操作。
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器容量允許誤差和測(cè)量重復(fù)性
| portant; word-wrap: break-word !important;">標(biāo)稱容量/μL | portant; word-wrap: break-word !important;">校準(zhǔn)點(diǎn)portant; word-wrap: break-word !important;">/μL | portant; word-wrap: break-word !important;">容量允許誤差 portant; word-wrap: break-word !important;">±(%) | portant; word-wrap: break-word !important;">測(cè)量重復(fù)性 portant; word-wrap: break-word !important;">≤(%) |
| portant; word-wrap: break-word !important;">20 | portant; word-wrap: break-word !important;">2 | portant; word-wrap: break-word !important;">12.0 | portant; word-wrap: break-word !important;">6.0 |
| portant; word-wrap: break-word !important;">10 | portant; word-wrap: break-word !important;">8.0 | portant; word-wrap: break-word !important;">4.0 | |
| portant; word-wrap: break-word !important;">20 | portant; word-wrap: break-word !important;">4.0 | portant; word-wrap: break-word !important;">2.0 | |
| portant; word-wrap: break-word !important;">50 | portant; word-wrap: break-word !important;">5 | portant; word-wrap: break-word !important;">8.0 | portant; word-wrap: break-word !important;">4.0 |
| portant; word-wrap: break-word !important;">25 | portant; word-wrap: break-word !important;">4.0 | portant; word-wrap: break-word !important;">2.0 | |
| portant; word-wrap: break-word !important;">50 | portant; word-wrap: break-word !important;">3.0 | portant; word-wrap: break-word !important;">1.5 | |
| portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">10 | portant; word-wrap: break-word !important;">8.0 | portant; word-wrap: break-word !important;">4.0 |
| portant; word-wrap: break-word !important;">50 | portant; word-wrap: break-word !important;">3.0 | portant; word-wrap: break-word !important;">1.5 | |
| portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">2.0 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">200 | portant; word-wrap: break-word !important;">20 | portant; word-wrap: break-word !important;">4.0 | portant; word-wrap: break-word !important;">2.0 |
| portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">2.0 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">200 | portant; word-wrap: break-word !important;">1.5 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">1000 | portant; word-wrap: break-word !important;">100 | portant; word-wrap: break-word !important;">2.0 | portant; word-wrap: break-word !important;">1.0 |
| portant; word-wrap: break-word !important;">500 | portant; word-wrap: break-word !important;">1.0 | portant; word-wrap: break-word !important;">1.0 | |
| portant; word-wrap: break-word !important;">1000 | portant; word-wrap: break-word !important;">1.0 | portant; word-wrap: break-word !important;">0.5 | |
| portant; word-wrap: break-word !important;">5000 | portant; word-wrap: break-word !important;">500 | portant; word-wrap: break-word !important;">1.0 | portant; word-wrap: break-word !important;">0.5 |
| portant; word-wrap: break-word !important;">2500 | portant; word-wrap: break-word !important;">0.5 | portant; word-wrap: break-word !important;">0.2 | |
| portant; word-wrap: break-word !important;">5000 | portant; word-wrap: break-word !important;">0.6 | portant; word-wrap: break-word !important;">0.2 |
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器快速校準(zhǔn)的方法
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">使用設(shè)備及物品：電子天平（測(cè)量范圍200g；分度值0.1mg）；燒杯（50mL）1個(gè)；燒杯（100mL）1個(gè)；定性濾紙若干小塊；超純水80mL；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">1.打開電子天平，空載調(diào)零；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">2.放入小燒杯待天平顯示穩(wěn)定后，按下歸零鍵歸零。給移液器上安裝相應(yīng)的吸液嘴，對(duì)移液器的槍頭進(jìn)行預(yù)洗；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">3.將移液器的容量調(diào)至被檢點(diǎn)。被檢點(diǎn)一般選擇最小，中間和最大容量值。可以是某一指定的容量點(diǎn)；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">4.使用移液器移取被檢容量的超純水至天平的小燒杯內(nèi)；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">5.待天平顯示穩(wěn)定后，馬上讀取并記錄此時(shí)電子天平顯示出的數(shù)值。記錄完畢將電子天平復(fù)零；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">6.重復(fù)5次執(zhí)行3-5條。記錄完畢，將移液器的容量調(diào)至下一被檢點(diǎn)繼續(xù)。被檢點(diǎn)遵循從小到大的原則進(jìn)行調(diào)整；
portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的評(píng)價(jià)標(biāo)準(zhǔn)
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portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">表一.移液器容量允許誤差和測(cè)量重復(fù)性
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portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的修正方法
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">1.將移液器調(diào)整至最小量程，按照校準(zhǔn)方法得到當(dāng)前的實(shí)際容量值，根據(jù)與標(biāo)示容量值的差值，通過移液器上的調(diào)準(zhǔn)旋鈕進(jìn)行調(diào)整；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">2.當(dāng)調(diào)整后測(cè)得的實(shí)際容量值與標(biāo)示容量值符合表1要求時(shí)，將移液器容量調(diào)整至中間量程繼續(xù)測(cè)量。中間量程符合要求時(shí)，再調(diào)整至最大量程繼續(xù)測(cè)量。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">3.如果中間量程或者最大量程有一個(gè)不符合表1要求時(shí)，則需重新調(diào)整至最小量程繼續(xù)修正。直到3個(gè)量程的值都符合表1要求為止。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">4.修正完畢的移液器，需重新進(jìn)行校準(zhǔn)。
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">5.最小量程低于10μL的，不進(jìn)行修正。如果最大量程和中間量程符合校準(zhǔn)要求的，繼續(xù)使用，否則返廠維修。
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portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">移液器的日常維護(hù)保養(yǎng)
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">1.如長(zhǎng)時(shí)間不使用，要把移液槍的量程調(diào)至最大值的刻度，使彈簧處于松弛狀態(tài)以保護(hù)彈簧；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">2.定期清潔移液器外壁，可以用95%酒精或60%的異丙醇，再用蒸餾水擦拭，自然晾干；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">3.使用時(shí)要檢查是否有漏液現(xiàn)象。方法是吸取液體后懸空垂直放置幾秒中，看看液面是否下降。如果漏液，則檢查吸液嘴是否匹配和彈簧活塞是否正常；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">4.當(dāng)移液器吸嘴內(nèi)有液體時(shí)，嚴(yán)禁將移液器水平或倒置放置，以防液體流入活塞室腐蝕移液器活塞；
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">5.使用正確的方法安裝吸頭，切記用力不能過猛，更不能采取剁吸頭的方法來進(jìn)行安裝；
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portant; word-wrap: break-word !important; font-size: 18px; color: rgb(0, 82, 255);">小結(jié)portant; word-wrap: break-word !important; font-family: 宋體; font-weight: bold; font-size: 20px; color: rgb(61, 167, 66);">
portant; word-wrap: break-word !important; font-size: 16px; line-height: 24px; font-family: 宋體;">需要提醒注意的是：移液器如果需要消毒可以用紫外照，或者用75%酒精擦拭，且不可近火呦。
