DetergentOUT™ GB-S10去污劑去除柱子和樹脂786-154

 DetergentOUT™ GB-S10去污劑去除柱子和樹脂去污劑在蛋白提取和樣本制備過程中是很重要的物質(zhì)，特別是研究疏水蛋白時。蛋白樣本中高濃度去污劑的存在能夠影響ELISA,IEF 和蛋白的蛋白酶消化，以及用質(zhì)譜進行分析時抑制肽的離子化。DetergentOUT™ GB-S10 樹脂能夠從蛋白水溶液和肽樣本中去除自由的，未結(jié)合的陰離子的，非離子或兩性離子的去污劑(e.g. SDS, Triton® X-100 或CHAPS)，同時對于下游進行的質(zhì)譜或其他技術(shù)的分析應用帶來的樣本丟失。DetergentOUT™ GB-S10 柱子在第三方的研究使用中表明完全兼容DI-QTOF 和 LC-MS/MS（請見參考文獻）。DetergentOUT™ GB-S10 柱子的使用顯著增加到了檢測到的肽光譜的數(shù)量。除此之外，DetergentOUT™ GB-S10 柱子對于去污劑有高度的結(jié)合容量，比如每毫升固定的樹脂可以結(jié)合6mg SDS 和14mg Triton® X-100。Figure 1: DetergentOUT™ GBS10 removes detergent & allows detection of peptide fragments by mass spectrometry. 500μgphosphorylase B was digested in solution & then the indicated amount of detergent was added. Samples were treated withDetergentOUT™ GBS10. Number of peptide spectra were determined as per the protocol of Alvarez, S. et al.A. No detergent, No DetergentOUT™ GBS10 B. 0.5% CHAPS, DetergentOUT™ GBS10 treatedC. 0.5% CHAPS, No DetergentOUT™ GBS10Figure 2: DetergentOUT™ GBS10 enhances mass spectrometry spectra. 5μg/μl protein mixture (BSA, cyctochrome Cand phosphorylase B) in water (Panel A) was supplemented with 0.5% CHAPS (Panel B and C). The CHAPS containing sample wastreated with DetergentOUT™ GBS10 and compared to an untreated sample (Panel C). Spectra were generated per Alvarez et al.參考文獻：Table 1: A comparison of the detergent removal rates and percentage protein recovery with DetergentOUT™ GBS10.Figure 3: DetergentOUT™ GBS10 retains ≤6mg SDS per ml settled resin. SDS solution was continuously applied toDetergentOUT™ GBS10 column. The graph depicts the amount of SDS detected in the flow- through. SDS was notdetected until fraction 7, so after 12mg SDS had been retained by the 2ml of DetergentOUT™ GB-S10 resin, resulting in a 6mg/mlsettled resin binding capacity.1. Alvarez, S. et al (2010) Poster presented as part of the 58th ASMS Conference on Mass2. Spectrometry and Allied Topics, May 23-27, 2010, Salt Lake City, Utah3. Sivakumar, S. et al (2007) J. Biol. Chem. 282: 73124. Urdaneta, S. et al (2006) J. Human Lact. 22: 615. Higgins, D. et al (2005) Anitmicrob. Agents Chemother. 49: 11276. Fisher, J. and Margulies, S. (2002) Am. J. Physiol. Lung Cell Mol. Physiol. 283: L7377. Baizman, E. et al (2000) Microbiology 146: 3129產(chǎn)品貨號 產(chǎn)品名稱 包裝786-154 DetergentOUT™ GB-S10-125 10 columns786-155 DetergentOUT™ GB-S10-800 10 columns786-156 DetergentOUT™ GB-S10-3000 10 columns786-157 DetergentOUT™ GB-S10-5000 10 columns786-159 DetergentOUT™ GB-S10 10ml resin

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